Standardized curcuminoid extract (Curcuma longa l.) decreases gene expression related to inflammation and interacts with associated microRNAs in human umbilical vein endothelial cells (HUVEC)

Food Funct.2012;3:1286–93

 

Chronic inflammation induced by pathogenic bacterial or viral infection, or other factors play a crucial role in the progression of atherosclerosis. Additionally, other pro-inflammatory mediators like intercellular adhesion molecules (ICAMs) and vascular cell adhesion molecule-1 (VCAM-1), and endothelial-selection (E-selectin) are known to be involved in causing endothelial dysfunction and tissue injury. Studies have discussed about the role of miRNAs (miRNA-126 and miRNA-146a), regulatory RNAs, during inflammatory process.

Studies have demonstrated effects of natural compounds, such as Curcuminoids on inflammation-involved miRNAs in conditions like cancer and apoptosis. However, protective role of Curcuminoids and involvement of miRNAs against chronic inflammation and related endothelial dysfunction is yet to be determined.

 

Objective:

To explicate involvement of miRNA-126 and miRNA-146a in the anti-inflammatory effects of Curcuminoids in human umbilical vein endothelial cells (HUVEC).

 

Study Design:

  • Cell viability assay was performed in the presence of Curcuminoids for 24 h
  • Intracellular reactive oxygen species (ROS) generation was determined by stimulating HUVEC with E. coli lipopolysaccharides (LPS) (1 µg/mL) after pre-treating with varying concentrations of Curcuminoids (0–10 mg/L) for 60 min
  • mRNA analysis was done on cells pre-treated with varying concentrations of Curcuminoids (0–5 mg/L) for 30 min. followed by LPS-challenge for 24 h, and MicroRNA analysis was carried out using qRT-PCR
  • Furthermore, western-blot analysis and multiplex bead assay were performed to evaluate protein expression

 

Results and Discussion:

  • Treatment with Curcuminoids at varying concentrations resulted no change in cell viability and showed protection against ROS generation as well
  • Curcuminoids treatment also down-regulated the LPS-challenge induced mRNA and protein levels of pro-inflammatory mediators, such as NF-ĸB, IL-6, TNF-α
  • In addition, LPS-induced up-regulation of miRNA 146a was inhibited by Curcuminoids. Similarly, Curcuminoids decreased the mRNA expression of VCAM-1 and ICAM-1

 

Conclusion:

It was concluded that Curcuminoids exhibit anti-inflammatory effects in HUVEC.

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